Antitumor effect and the distribution of iron in the tumor and blood serum during magnetic nanotherapy of Walker-256 carcinosarcoma

Summary. Magnetic nanotherapy by the magnetosensitive nanocomplex (MNC) comprised of Fe₃O₄ nanoparticles and doxorubicin with a hysteresis loop area of 1135.4 erg/g has initiated greater growth inhibition factor of carcinosarcoma Walker-256 on 23% as compared to the MNC with lesser hysteresis loop area of 136.3 erg/g. The content of free iron complexes was minimal in carcinosarcoma Wal­ker-256 after magnetic nanotherapy by MNC with a large hysteresis loop area, and the maximum level of free iron was observed in animals after exposure to conventional doxorubicin. The concentration of iron ions in the blood serum increased in 2.2 times on the average in all studied groups, regardless of received treatment, as compared to the control group and animals without tumors. The concentration of uric acid, alanine aminotransferase, alkaline phosphatase, and creatinine in the serum of the animals with the greatest antitumor effect of magnetic nanotherapy did not statistically differed from the animals treated by conventional doxorubicin. Obtained results testified that magnetic nanotherapy did not initiated additional overall toxicity. This opens up the opportunities for further development of the technology for controlled local toxicity within the allowable dose components MNC.