Study of the influence of humoral factors of blood of patients with brain gliomas on the subpopulation of lymphocytes in the reaction of lymphocytes blast transformation test
Lisyaniy N.I.1, Gnedkova I.O.2, Belskay L.M.
- 1SI «Institute of Neurosurgery named after acad. A.P. Romodanov, NAMS of Ukraine», Kyiv
- 2SI «Institute of Neurosurgery named after acad. A.P. Romodanov of the NAMS of Ukraine», Kyiv
Summary. In malignant gliomas of the brain, multidirectional immune disorders are noted, as well as by synthesis of tumor cells of immunosuppressive humoral factors. The production and mechanism of the effect of humoral tumor factors on immune cells have been studied poorly. The purpose of the study was to study the effect of blood sera in patients with brain gliomas of varying degrees of anaplasia on the proliferative activity of lymphocytes in the reaction of lymphocytes blast transformation (RLBT) test. Blood serum of 26 patients with brain gliomas were divided into anaplasia and histology by 4 groups and studied in the RLBT test with phytohemagglutinin (PHA). Accounting RLBT was carried out morphologically, the number of subpopulations of lymphocytes was determined using monoclonal CD3, 4, 8, 16, 20 antibodies on a flow cytofluorimeter. It has been established that sera from patients with gliomas of grade III–IV anaplasia caused statistically significant inhibition of the RLBT reaction and proliferation of CD4 and CD8 lymphocytes. The CD16 and CD20 subpopulations of the lymphocytes did not change with the action of these blood sera. Blood serum of patients with gliomas of the I–II degree of anaplasia did not inhibit the RLBT reaction and did not affect the proliferative capacity of lymphocytes. In the blood of patients with brain gliomas of III–IV degrees of anaplasia, there are factors inhibiting the activation of CD4 and CD8 subpopulations of lymphocytes in the RLBT test with PHA, which responsible for specific antitumor immunity.
Leave a comment